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mini transblot electrophoretic transfer cell  (Bio-Rad)


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    Structured Review

    Bio-Rad mini transblot electrophoretic transfer cell
    Mini Transblot Electrophoretic Transfer Cell, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mini+transblot+electrophoretic+transfer+cell/Mini-PROTEAN+Tetra+Vertical+Electrophoresis+Cell/pmc12008219-189-22-26
    Average 93 stars, based on 104 article reviews
    mini transblot electrophoretic transfer cell - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Multidrug resistance transporter-1 dysfunction perturbs meiosis and Ca2+ homeostasis in oocytes
    Article Snippet: PageRulerTM Prestained Protein Ladder (Cat. #26616m, Fisher Scientific) was used to identify protein kDa. .. The gels were then run at 125 V for 60 min. Gels were then transferred to a PVDF membrane activated with methanol using the Mini TransBlot Electrophoretic Transfer Cell (Bio-Rad) for 2 h at 100 V. Membranes were then blocked with 5% (w/v) milk/TBS-T for 1 h and then were incubated with the primary antibody overnight at 4°C. .. Primary antibodies used were as follows: rabbit antiSirt1 (Cat. #9475); rabbit anti-Sirt3 (Cat. #5490); rabbit antiSirt5 (Cat. #8779); rabbit anti-Sirt6 (Cat. #12486); rabbit anti-Sirt7 (Cat #5360) (Cell Signaling Technology) at 1:500 in TBS-T. Due to the similar molecular weights of GAPDH (our loading control) with most sirtuins, the membranes were cut into strips, stripped using of ROTI Stripping Puffer (#0083.1, Roth, Karlsruhe, Baden-Württemberg, Germany) in a water bath rocking at 56°C for 30 min and washed with TBS-T and imaged to ensure there were no residual sirtuin bands.

    Article Title: The mTORC1-G9a-H3K9me2 axis negatively regulates autophagy in fatty acid-induced hepatocellular lipotoxicity.
    Article Snippet: .. Protein samples were resolved on 7 to 18% SDS gels at 100 V. Proteins were electrotransferred to polyvinylidene difluoride membrane (GE Healthcare Life Science) using Bio-Rad Mini Transblot Electrophoretic transfer Cell. .. Membranes were blocked in 5% fat-free milk in 50 mM Tris, pH 8.0 with 150 mM sodium chloride and 0.1% Tween-20 for 2 h. Rabbit: anti-LC3II, anti-Beclin1, anti-Atg7, anti-pP70S6 kinase, antiP70S6 kinase, anti-G9a, anti-pAMPK-Thr172, anti-p4-EBP1, anti-4-EBP1 (Cell Signalling Technology), anti-SQSTM1/p62 (Santa Cruz Biotechnologies), mouse: anti-β-actin (Sigma– Aldrich), antibodies were used as primary antibodies in 3% BSA overnight at 4 C. Anti-H3K9me2, anti-H3, and anti-Pol II antibodies obtained from abcam were used for ChIP experiments.

    Article Title: Bacterial inhibitors
    Article Snippet: P. gingivalis cells were lysed using BUGBUSTER® Protein Extraction Reagent (EMD Millipore, Darmstadt, Germany), and the protein concentration determined using a Bio-Rad protein assay (Bio-Rad). .. Lysates (0.5 μg) were separated by 12% sodium SDS-PAGE, and transferred to nitrocellulose membranes (Invitrogen, Carlsbad, Calif.) with a Mini Transblot Electrophoretic transfer cell (Bio-Rad) at 100 V for 1 h. The membrane was blocked with 3% BSA in PBS for 1 h and incubated with polyclonal anti-FimA, anti-Mfa1, anti-HGP44 (a C-terminal adhesin domain of gingipains), or anti-PGN-0128 antibodies diluted 1:1,000 for 1 h. After washing with PBS, the membrane was incubated with anti-rabbit horseradish peroxidase-conjugated secondary antibodies for 1 h. The proteins were visualized using enhanced chemiluminescence (GE Healthcare Bio-Sciences Corp, Pittsburgh, Pa.) and measured using a semi-quantitative Western blot technique with ImageJ software (NIH, Bethesda, Md.). ..

    Incubation:

    Article Title: Multidrug resistance transporter-1 dysfunction perturbs meiosis and Ca2+ homeostasis in oocytes
    Article Snippet: PageRulerTM Prestained Protein Ladder (Cat. #26616m, Fisher Scientific) was used to identify protein kDa. .. The gels were then run at 125 V for 60 min. Gels were then transferred to a PVDF membrane activated with methanol using the Mini TransBlot Electrophoretic Transfer Cell (Bio-Rad) for 2 h at 100 V. Membranes were then blocked with 5% (w/v) milk/TBS-T for 1 h and then were incubated with the primary antibody overnight at 4°C. .. Primary antibodies used were as follows: rabbit antiSirt1 (Cat. #9475); rabbit anti-Sirt3 (Cat. #5490); rabbit antiSirt5 (Cat. #8779); rabbit anti-Sirt6 (Cat. #12486); rabbit anti-Sirt7 (Cat #5360) (Cell Signaling Technology) at 1:500 in TBS-T. Due to the similar molecular weights of GAPDH (our loading control) with most sirtuins, the membranes were cut into strips, stripped using of ROTI Stripping Puffer (#0083.1, Roth, Karlsruhe, Baden-Württemberg, Germany) in a water bath rocking at 56°C for 30 min and washed with TBS-T and imaged to ensure there were no residual sirtuin bands.

    Article Title: Bacterial inhibitors
    Article Snippet: P. gingivalis cells were lysed using BUGBUSTER® Protein Extraction Reagent (EMD Millipore, Darmstadt, Germany), and the protein concentration determined using a Bio-Rad protein assay (Bio-Rad). .. Lysates (0.5 μg) were separated by 12% sodium SDS-PAGE, and transferred to nitrocellulose membranes (Invitrogen, Carlsbad, Calif.) with a Mini Transblot Electrophoretic transfer cell (Bio-Rad) at 100 V for 1 h. The membrane was blocked with 3% BSA in PBS for 1 h and incubated with polyclonal anti-FimA, anti-Mfa1, anti-HGP44 (a C-terminal adhesin domain of gingipains), or anti-PGN-0128 antibodies diluted 1:1,000 for 1 h. After washing with PBS, the membrane was incubated with anti-rabbit horseradish peroxidase-conjugated secondary antibodies for 1 h. The proteins were visualized using enhanced chemiluminescence (GE Healthcare Bio-Sciences Corp, Pittsburgh, Pa.) and measured using a semi-quantitative Western blot technique with ImageJ software (NIH, Bethesda, Md.). ..

    SDS Page:

    Article Title: Oncolytic virotherapy provides a potent therapy option for squamous bladder cancer
    Article Snippet: Protein concentrations of lysis supernatants were quantified using PierceTM BCA Protein Assay kit (23225, ThermoFisher). .. Protein lysates were separated by SDS-PAGE using Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories) and transferred to PVDF membranes (10600001, GE-Healthcare) using Mini-TransBlot Electrophoretic Transfer Cell (Bio-Rad Laboratories). .. Membranes were incubated in 5% w/v nonfat dry milk blocking buffer for 1 h followed by incubation with primary antibodies, anti-YB-1 (1:1000, ab76149, Abcam), anti-GAPDH (1:1000, #2118, Cell Signaling Technology), overnight at 4 °C.

    Article Title: Oncolytic virotherapy provides a potent therapy option for squamous bladder cancer.
    Article Snippet: Protein concentrations of lysis supernatants were quantified using PierceTM BCA Protein Assay kit (23225, ThermoFisher). .. Protein lysates were separated by SDS-PAGE using Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories) and transferred to PVDF membranes (10600001, GE-Healthcare) using Mini-TransBlot Electrophoretic Transfer Cell (Bio-Rad Laboratories). .. Membranes were incubated in 5% w/v nonfat dry milk blocking buffer for 1 h followed by incubation with primary antibodies, anti-YB-1 (1:1000, ab76149, Abcam), anti-GAPDH (1:1000, #2118, Cell Signaling Technology), overnight at 4 °C.

    Electrophoresis:

    Article Title: Oncolytic virotherapy provides a potent therapy option for squamous bladder cancer
    Article Snippet: Protein concentrations of lysis supernatants were quantified using PierceTM BCA Protein Assay kit (23225, ThermoFisher). .. Protein lysates were separated by SDS-PAGE using Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories) and transferred to PVDF membranes (10600001, GE-Healthcare) using Mini-TransBlot Electrophoretic Transfer Cell (Bio-Rad Laboratories). .. Membranes were incubated in 5% w/v nonfat dry milk blocking buffer for 1 h followed by incubation with primary antibodies, anti-YB-1 (1:1000, ab76149, Abcam), anti-GAPDH (1:1000, #2118, Cell Signaling Technology), overnight at 4 °C.

    Article Title: Oncolytic virotherapy provides a potent therapy option for squamous bladder cancer.
    Article Snippet: Protein concentrations of lysis supernatants were quantified using PierceTM BCA Protein Assay kit (23225, ThermoFisher). .. Protein lysates were separated by SDS-PAGE using Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories) and transferred to PVDF membranes (10600001, GE-Healthcare) using Mini-TransBlot Electrophoretic Transfer Cell (Bio-Rad Laboratories). .. Membranes were incubated in 5% w/v nonfat dry milk blocking buffer for 1 h followed by incubation with primary antibodies, anti-YB-1 (1:1000, ab76149, Abcam), anti-GAPDH (1:1000, #2118, Cell Signaling Technology), overnight at 4 °C.

    Western Blot:

    Article Title: Bacterial inhibitors
    Article Snippet: P. gingivalis cells were lysed using BUGBUSTER® Protein Extraction Reagent (EMD Millipore, Darmstadt, Germany), and the protein concentration determined using a Bio-Rad protein assay (Bio-Rad). .. Lysates (0.5 μg) were separated by 12% sodium SDS-PAGE, and transferred to nitrocellulose membranes (Invitrogen, Carlsbad, Calif.) with a Mini Transblot Electrophoretic transfer cell (Bio-Rad) at 100 V for 1 h. The membrane was blocked with 3% BSA in PBS for 1 h and incubated with polyclonal anti-FimA, anti-Mfa1, anti-HGP44 (a C-terminal adhesin domain of gingipains), or anti-PGN-0128 antibodies diluted 1:1,000 for 1 h. After washing with PBS, the membrane was incubated with anti-rabbit horseradish peroxidase-conjugated secondary antibodies for 1 h. The proteins were visualized using enhanced chemiluminescence (GE Healthcare Bio-Sciences Corp, Pittsburgh, Pa.) and measured using a semi-quantitative Western blot technique with ImageJ software (NIH, Bethesda, Md.). ..



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